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HA-Tag Mouse mAb

EM1002

規(guī)格：	價格：
50μL	￥1280.00
100μL	￥1980.00

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Overview

Product name:	HA-Tag Mouse mAb
Source:	Mouse
Dilutions:	WB 1:5,000 IP 1:200 IF 1:1,000
Immunogen:	Synthetic Peptide
Storage:	PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid?repeated?freeze-thaw?cycles.
Clonality:	Monoclonal
Isotype:	IgG1
Concentration:	1mg/mL
Background:	Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein.HA tag antibody is a highly sensitive and affinity monoclonal antibody applicable to HA-tagged fusion protein detection.

IP antibody use：5ug HA Mouse IgG1 per ml Lysate，WB 1:5000 1、untransfected 293 cell lysate 2、transfected 293 cell lysate with HA-tag fusion protein 3、IP(untransfted 293+anti-HA mAb+Protein G agarose) 4、IP (transfected 293+ normal Mouse IgG+Protein G ag
IF analysis of 293 cells transfected with a HA-tag protein, using ELKbio anti-HATag Mouse mAb at a 1:2000 dilution (blue DAPI, red anti-HA)
0.5ug HA fusion protein+ Primary antibody dilution at 1:10,000.

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